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mouse anti pan gap43  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti pan gap43
    Mouse Anti Pan Gap43, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+gap43/GAP-43+Antibody/10__1523_slash_jneurosci__0701___24__2024-90-35-38
    Average 95 stars, based on 340 article reviews
    mouse anti pan gap43 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Guanidinoacetic acid supplementation improves intestinal morphology, mucosal barrier function of broilers subjected to chronic heat stress.
    Article Snippet: Studies have shown that serum response factor is beneficial for axonal regeneration of peripheral nerves.. However, its role after central nervous system injury remains unclear.. In this study, we established a rat model of T9–T10 spinal cord transection injury.

    Article Title: Anti-neuroinflammatory and neurotrophic effects of combined therapy with annexin II and Reg-2 on injured spinal cord.
    Article Snippet: .. Immunofluorescence Labeling The sections were pretreated with the same method as described above, incubated with primary polyclonal rabbit anti160KD NF-M primary antibody (1: 1,000), anti-MPO (1: 100, Santa Cruz Biotechnology, Calif., USA), anti-GFAP (1: 200) and monoclonal antibodies including mouse anti-GAP43, CD45, CD68/ED1 (1: 100; Santa Cruz Biotechnology) overnight at 4 ° C, then washed with PBS and incubated with 1: 200 TRITC or FITCconjugated goat anti-rabbit/mouse IgG secondary antibodies for 1 h at 37 ° C (Invitrogen, Carlsbad, Calif., USA). .. The sections were finally coverslipped with antifade Gel/Mount aqueous mounting media (SouthernBiotech, Birmingham, Ala., USA).

    Article Title: Serum response factor promotes axon regeneration following spinal cord transection injury
    Article Snippet: The tissue was circled by an immunohistochemical pen (Biosharp, Hefei, China, Cat# BC003) and rinsed with phosphate buffered saline, and then 5% goat serum (ZSGB-BIO, Beijing, China, ZLI-9022) was added (37°C for 30 minutes) to block non-specific binding. .. Subsequently, mouse anti-GAP43 (1:200, Santa Cruz Biotechnology, Cat# sc-17790, RRID: AB_627660) and rabbit anti-Nogo A (1:200, Invitrogen, Cat# 36-6600, RRID: AB_2533273) were added to the tissue sections and incubated at 4°C overnight. .. After the sections were rinsed with phosphate buffered saline three times, secondary antibody goat anti-mouse (Alexa Fluor 488, 1:100, Invitrogen, Cat# A-11094, RRID: AB_221544) and goat anti-rabbit (Alexa Fluor 594, 1:200, ZSGB-BIO, Cat# ZF-0516) were added to the sections (37°C for 2 hours).

    Bioprocessing:

    Article Title: The Neuroprotective Effects of Reg-2 Following Spinal Cord Transection Injury
    Article Snippet: .. The sections were per-treated according to the method described above and incubated with primary polyclonal rabbit anti-160KD NF-M primary antibodies (1:1,000, Neuromics, MN), anti-GFAP (1:200, Thermo Fisher Scientific, MA), and monoclonal antibodies including mouse anti-GAP43, CD45, and CD68/ED1 (1:100; Santa Cruz Biotechnology, CA) overnight at 4 C. The sections were then washed with PBS and incubated with 1:200 TRITCor FITC-conjugated goat anti-rabbit/mouse IgG secondary antibodies for 1 hr at 37 C (Invitrogen, CA). .. The sections were finally coverslipped with antifade Gel/Mount aqueous mounting media (SouthernBiotech, AL).

    Article Title: Anti-neuroinflammatory and neurotrophic effects of combined therapy with annexin II and Reg-2 on injured spinal cord.
    Article Snippet: .. Immunofluorescence Labeling The sections were pretreated with the same method as described above, incubated with primary polyclonal rabbit anti160KD NF-M primary antibody (1: 1,000), anti-MPO (1: 100, Santa Cruz Biotechnology, Calif., USA), anti-GFAP (1: 200) and monoclonal antibodies including mouse anti-GAP43, CD45, CD68/ED1 (1: 100; Santa Cruz Biotechnology) overnight at 4 ° C, then washed with PBS and incubated with 1: 200 TRITC or FITCconjugated goat anti-rabbit/mouse IgG secondary antibodies for 1 h at 37 ° C (Invitrogen, Carlsbad, Calif., USA). .. The sections were finally coverslipped with antifade Gel/Mount aqueous mounting media (SouthernBiotech, Birmingham, Ala., USA).

    Immunofluorescence:

    Article Title: Anti-neuroinflammatory and neurotrophic effects of combined therapy with annexin II and Reg-2 on injured spinal cord.
    Article Snippet: .. Immunofluorescence Labeling The sections were pretreated with the same method as described above, incubated with primary polyclonal rabbit anti160KD NF-M primary antibody (1: 1,000), anti-MPO (1: 100, Santa Cruz Biotechnology, Calif., USA), anti-GFAP (1: 200) and monoclonal antibodies including mouse anti-GAP43, CD45, CD68/ED1 (1: 100; Santa Cruz Biotechnology) overnight at 4 ° C, then washed with PBS and incubated with 1: 200 TRITC or FITCconjugated goat anti-rabbit/mouse IgG secondary antibodies for 1 h at 37 ° C (Invitrogen, Carlsbad, Calif., USA). .. The sections were finally coverslipped with antifade Gel/Mount aqueous mounting media (SouthernBiotech, Birmingham, Ala., USA).

    Labeling:

    Article Title: Anti-neuroinflammatory and neurotrophic effects of combined therapy with annexin II and Reg-2 on injured spinal cord.
    Article Snippet: .. Immunofluorescence Labeling The sections were pretreated with the same method as described above, incubated with primary polyclonal rabbit anti160KD NF-M primary antibody (1: 1,000), anti-MPO (1: 100, Santa Cruz Biotechnology, Calif., USA), anti-GFAP (1: 200) and monoclonal antibodies including mouse anti-GAP43, CD45, CD68/ED1 (1: 100; Santa Cruz Biotechnology) overnight at 4 ° C, then washed with PBS and incubated with 1: 200 TRITC or FITCconjugated goat anti-rabbit/mouse IgG secondary antibodies for 1 h at 37 ° C (Invitrogen, Carlsbad, Calif., USA). .. The sections were finally coverslipped with antifade Gel/Mount aqueous mounting media (SouthernBiotech, Birmingham, Ala., USA).

    Tissue:

    Article Title: Serum response factor promotes axon regeneration following spinal cord transection injury
    Article Snippet: The tissue was circled by an immunohistochemical pen (Biosharp, Hefei, China, Cat# BC003) and rinsed with phosphate buffered saline, and then 5% goat serum (ZSGB-BIO, Beijing, China, ZLI-9022) was added (37°C for 30 minutes) to block non-specific binding. .. Subsequently, mouse anti-GAP43 (1:200, Santa Cruz Biotechnology, Cat# sc-17790, RRID: AB_627660) and rabbit anti-Nogo A (1:200, Invitrogen, Cat# 36-6600, RRID: AB_2533273) were added to the tissue sections and incubated at 4°C overnight. .. After the sections were rinsed with phosphate buffered saline three times, secondary antibody goat anti-mouse (Alexa Fluor 488, 1:100, Invitrogen, Cat# A-11094, RRID: AB_221544) and goat anti-rabbit (Alexa Fluor 594, 1:200, ZSGB-BIO, Cat# ZF-0516) were added to the sections (37°C for 2 hours).



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    Santa Cruz Biotechnology mouse anti pan gap43
    Implantation of GA@PDA scaffold scavenged the accumulated ROS and down-regulated inflammation after ONC. (a) Fluorescence images of ROS in the optic nerve labeled with the fluorescence probe DCF (green) 3 weeks post scaffold implantation. DAPI (blue) was used to stain nuclei. (b-d) Immunofluorescence staining images and mean fluorescence intensity <t>of</t> <t>IL-1β</t> and TNF-α in the retina 3 weeks post scaffold implantation ( n = 5). (e-h) Western blot analysis of TNF-α, IL-1β and IL-6 in each group ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, no significance
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    Image Search Results


    Implantation of GA@PDA scaffold scavenged the accumulated ROS and down-regulated inflammation after ONC. (a) Fluorescence images of ROS in the optic nerve labeled with the fluorescence probe DCF (green) 3 weeks post scaffold implantation. DAPI (blue) was used to stain nuclei. (b-d) Immunofluorescence staining images and mean fluorescence intensity of IL-1β and TNF-α in the retina 3 weeks post scaffold implantation ( n = 5). (e-h) Western blot analysis of TNF-α, IL-1β and IL-6 in each group ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, no significance

    Journal: Journal of Nanobiotechnology

    Article Title: Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment

    doi: 10.1186/s12951-024-02962-y

    Figure Lengend Snippet: Implantation of GA@PDA scaffold scavenged the accumulated ROS and down-regulated inflammation after ONC. (a) Fluorescence images of ROS in the optic nerve labeled with the fluorescence probe DCF (green) 3 weeks post scaffold implantation. DAPI (blue) was used to stain nuclei. (b-d) Immunofluorescence staining images and mean fluorescence intensity of IL-1β and TNF-α in the retina 3 weeks post scaffold implantation ( n = 5). (e-h) Western blot analysis of TNF-α, IL-1β and IL-6 in each group ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, no significance

    Article Snippet: The sections were washed three times in 10 mM PBS, blocked with PBS containing 0.3% Triton X-100 and 10% normal goat serum at RT for 1 h, and incubated overnight with the antibodies, including rabbit polyclonal anti-TNF-α antibody (1:500, Bioss, bs-10802R), mouse monoclonal anti-IL-1β antibody (1:500, Proteintech, 66737-1-Ig), rabbit polyclonal anti-GAP-43 antibody (1:200, Abcam, ab75810), mouse monoclonal anti-βIII Tubulin antibody (1:500, Promega, G7121), mouse monoclonal anti-CD206 antibody (1:500, abcam, ab64693), mouse monoclonal anti-CD86 antibody (1:500, abcam, ab220188), Rabbit monoclonal anti-iNOS antibody (1:500, abcam, ab178945) and Rabbit polyconal anti- Arg-1 antibody (1:500, proteintech, 16001-1-AP).

    Techniques: Fluorescence, Labeling, Staining, Immunofluorescence, Western Blot